Multiplex Legation-Dependent Probe Amplification (MLPA) is a method for the detection of multiple chromosomal aberrations in a single experiment. In one reaction over 40 different probe sets are analyzed. For a reliable work flow tools are needed for administrative support, data management, normalization, visualization, reporting and interpretation. We developed a data management system for monitoring and interpreting the MLPA data stream from experimental setup to analysis, quality control and visualization. A statistical approach is applied for the normalization and analysis of large series of MLPA traces, making use of multiple control samples and internal controls. Results are visualized in plots with information about sample replicates, normalization settings, and sample characteristics. The system, MLPAINTER, for MLPA interpretation combines an Access database and a Delphi front end. MLPAINTER is freely available.
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